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Western blot analysis of ZBED3 in A-20 cell lysate with ZBED3 antibody at 1 μg/ml.
Western blot analysis of ZBED3 in A-20 cell lysate with ZBED3 antibody at 1 μg/ml.
Trial Size

ZBED3 Antibody

CATALOG NUMBER: 5123

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZBED3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ZBED3.
The immunogen is located within the first 50 amino acids of ZBED3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 28 kDa
Purification:
ZBED3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBED3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBED3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBED3
Additional Names:
ZBED3 Antibody: Zinc finger BED domain-containing protein 3, Axin-interacting protein
Protein Accession Number:
NP_115743
Protein Gi Number:
14150185
Ncbi Gene Id Number:
84327
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBED3 Antibody: Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression.
Background Reference 1:
Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem.2009; 284:6683-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5123

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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