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Western blot analysis of extracts of various cell lines, using ZBTB17 Antibody (23-600) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using ZBTB17 antibody (23-600) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ZBTB17 antibody (23-600) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ZBTB17 antibody (23-600) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ZBTB17 antibody (23-600) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ZBTB17 Antibody (23-600) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ZBTB17 Antibody

CATALOG NUMBER: 23-600

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 121-360 of human ZBTB17 (NP_003434.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
B cells
Positive Control 2:
U-87MG
Positive Control 3:
Hela
Predicted Molecular Weight:
Observed: 103kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ZBTB17
Additional Names:
Zinc finger and BTB domain-containing protein 17, Myc-interacting zinc finger protein 1, Miz-1, Zinc finger protein 151, Zinc finger protein 60, ZBTB17, MIZ1, ZNF151, ZNF60
Ncbi Gene Id Number:
7709
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-600

List Size:
100 uL

List Price:

$436.00

Datasheet
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