ZBTB33 Antibody

CATALOG NUMBER: 28-957

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZBTB33.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZBTB33 antibody can be used for detection of ZBTB33 by ELISA at 1:312500. ZBTB33 antibody can be used for detection of ZBTB33 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
74 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ZBTB33 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ZBTB33
Additional Names:
ZBTB33, ZNF348, ZNF-kaiso
Protein Accession Number:
NP_006768
Protein Gi Number:
5803229
Ncbi Gene Id Number:
10009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB33 is a transcriptional regulator with bimodal DNA-binding specificity. ZBTB33 binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway. It may also activate transcription of a subset of target genes by the recruitment of CTNND2.
Background Reference 1:
Defossez, P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-957

List Size:
100 ul

List Price:

$519.00

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