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Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/mL.
Immunohistochemistry of ZBTB4 in human brain tissue with ZBTB4 antibody at 2.5 μg/mL.
Immunofluorescence of ZBTB4 in human brain tissue with ZBTB4 antibody at 20 μg/mL.
Western blot analysis of ZBTB4 in SK-N-SH cell lysate with ZBTB4 antibody at 1 μg/mL.
Trial Size

ZBTB4 Antibody

CATALOG NUMBER: 6121

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%)
Immunogen:
ZBTB4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human ZBTB4.
The immunogen is located within amino acids 790 - 840 of ZBTB4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZBTB4 antibody can be used for detection of ZBTB4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZBTB4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBTB4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBTB4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBTB4
Additional Names:
ZBTB4 Antibody: ZNF903, KAISO-L1, KIAA1538, Zinc finger and BTB domain-containing protein 4, KAISO-like zinc finger protein 1
Protein Accession Number:
EAW90185
Protein Gi Number:
192807286
Ncbi Gene Id Number:
57659
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB4 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.
Background Reference 1:
Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903.
Background Reference 2:
Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81.
Background Reference 3:
Weber A, Marquardt J, Elzi D, et al. Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation. EMBO J.2008; 27:1563-74.
Background Reference 4:
Yamada D, Perez-Torrado R, Filion G, et al. The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4. Oncogene2009; 28:2535-44.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6121

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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