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Western blot analysis of ZBTB7B Antibody Pab in mouse brain tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ZBTB7B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ZBTB7B Antibody Pab in mouse brain tissue lysates (35ug/lane)

ZBTB7B Antibody

CATALOG NUMBER: 62-265

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the Central region of human ZBTB7B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBTB7B
Additional Names:
Zinc finger and BTB domain-containing protein 7B, Krueppel-related zinc finger protein cKrox, hcKrox, T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 15, Zinc finger protein 67 homolog, Zfp-67, Zinc finger protein 857B, Zinc finger protein Th-POK, ZBTB7B, ZBTB15, ZFP67, ZNF857B
Protein Accession Number:
O15156
Ncbi Gene Id Number:
51043
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.
Background Reference 1:
Galera,P., Proc. Natl. Acad. Sci. U.S.A. 91 (20), 9372-9376 (1994)
Background Reference 2:
Widom,R.L., Gene 198 (1-2), 407-420 (1997)
Background Reference 3:
Heegaard,A.M., J. Bone Miner. Res. 12 (12), 2050-2060 (1997)
Background Reference 4:
Widom,R.L., Matrix Biol. 20 (7), 451-462 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-265

List Size:
400 ul

List Price:

$495.00

Datasheet
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