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Western blot analysis of ZBTB9 in mouse heart tissue lysate with ZBTB9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 2.5 μg/mL.
Immunofluorescence of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 20 μg/mL.
Western blot analysis of ZBTB9 in mouse heart tissue lysate with ZBTB9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZBTB9 Antibody

CATALOG NUMBER: 6131

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ZBTB9 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ZBTB9.
The immunogen is located within the first 50 amino acids of ZBTB9.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZBTB9 antibody can be used for detection of ZBTB9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to not cross-react with other ZBTB protein family members.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
ZBTB9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZBTB9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZBTB9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZBTB9
Additional Names:
ZBTB9 Antibody: ZNF919, Zinc finger and BTB domain-containing protein 9
Protein Accession Number:
NP_689948
Protein Gi Number:
23308693
Ncbi Gene Id Number:
221504
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZBTB9 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB9, also known as ZNF919, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB9, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB9 may act as a transcriptional repressor or be involved in tumorigenesis.
Background Reference 1:
Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol. 2006; 26:169-81.
Background Reference 2:
Qu H, Qu D, Chen F, et al. ZBTB7 overexpression contributes to malignancy in breast cancer. Cancer Invest. 2010; 28:672-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6131

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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