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<strong>Figure 1. WB Validation in Human Lung </strong><br>Loading: 15 μg of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 μg/mL Lane 2: 4 μg/mL
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3. Western Blot Validation in Rat Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: ZC3H12A 9547, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with 9547 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5. Immunohistochemistry Validation of ZC3H12A in Human Testis</strong><br>Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse Liver</strong><br>Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin
<strong>Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat Brain</strong><br>Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (9547) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1. WB Validation in Human Lung
Loading: 15 μg of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 μg/mL Lane 2: 4 μg/mL
New
Trial Size

ZC3H12A (CT) Antibody

CATALOG NUMBER: 9547

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chimpanzee (100%); Monkey (100%); Pig (79%); Chicken (83%)
Immunogen:
Anti-ZC3H12A antibody (9547) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A.

The immunogen is located within the last 50 amino acids of ZC3H12A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
WB: 2 μg mL; IF: 10 μg mL; IHC: 1 μg mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1701-01 - Human Lung Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 66 kD
Observed: 66 kD
Isoforms:
Human ZC3H12A has one isoform (599aa, 65.7kD). Mouse has one isoform (596aa, 65.6kD). Rat has one isoform (596aa, 65.9kD). 9547 can detect ZC3H12A in human, mouse, and rat.
Purification:
ZC3H12A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZC3H12A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZC3H12A
Additional Names:
ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocyte chemotactic protein-induced protein 1, MCP-induced protein 1, MCPIP-1, Zinc finger CCCH domain-containing protein 12A
Protein Accession Number:
Q5D1E7.2
Protein Gi Number:
190485853
Ncbi Gene Id Number:
80149
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZC3H12A Antibody: ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis. ZC3H12A inhibits the Wnt/beta-catenin signaling pathway activity and modulates epithelial-mesenchymal transition during clear cell renal cell carcinoma progression by targeting miRNAs. ZC3H12A ribonuclease can bind and cleave AURKA mRNA in MYCN-amplified neuroblastoma cells. ZC3H12A is a novel link between diabetogenic conditions and impaired insulin secretory capacity. ZC3H12A overexpression in human neuroblastoma cell lines causes cell-cycle arrest by G1/S checkpoint block.
Background Reference 1:
Gorka et al. Oncogene. 2021; 40(50):6720-6735
Background Reference 2:
Nowak et al. RNA Biol. 2021; 18(1):144-156
Background Reference 3:
Tyka et al. Biochim Biophys Acta Mol Basis Dis. 2021; 1867(10):166199
Background Reference 4:
Boratyn et al. J Cell Biochem. 2020; 121(5-6);3406-3425

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9547

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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