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Western blot analysis of ZC3H12D in human spleen tissue lysate with ZC3H12D antibody at 1 μg/mL.
Western blot analysis of ZC3H12D in human spleen tissue lysate with ZC3H12D antibody at 1 μg/mL.
Trial Size

ZC3H12D Antibody

CATALOG NUMBER: 5679

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ZC3H12D antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZC3H12D.
The immunogen is located within amino acids 280 - 330 of ZC3H12D.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZC3H12D antibody can be used for detection of ZC3H12D by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 58 kDa
Observed: 57 kDa
Purification:
ZC3H12D Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZC3H12D Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZC3H12D antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZC3H12D
Additional Names:
ZC3H12D Antibody: TFL, p34, MCPIP4, C6orf95, dJ281H8.1, TFL, Probable ribonuclease ZC3H12D, MCP-induced protein 4
Protein Accession Number:
EAW47801
Protein Gi Number:
119568186
Ncbi Gene Id Number:
340152
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZC3H12D Antibody: ZC3H12D, also known as MCPIP4, is a member of a family of novel CCCH-zinc finger proteins that includes ZC3H12A, a protein that is thought to be involved in macrophage activation, host immunity and inflammatory diseases. ZC3H12D is highly expressed in spleen, intestine, and colon. Similar to ZC3H12A and ZC3H12C, ZC3H12D expression in murine macrophages is also increased after treatment with lipopolysaccharide (LPS), although not to the same level as ZC3H12A. This pattern of expression was not duplicated in human-derived macrophages, suggesting it also may play a different role in host immunity and inflammatory response.
Background Reference 1:
Zhou L, Azfer A, Niu J, et al. Monocyte chemoattractant protein-1 induces a novel transcription factor that causes cardiac myocyte apoptosis and ventricular dysfunction. Circ. Res.2006; 98:1177-85.
Background Reference 2:
Liang J, Wang J, Azfer A, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J. Biol. Chem.2008; 283:6337-46.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5679

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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