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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

ZC3H8 Antibody

CATALOG NUMBER: 55-863

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ZC3H8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human ZC3H8.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZC3H8
Additional Names:
Zinc finger CCCH domain-containing protein 8, ZC3H8, ZC3HDC8
Protein Accession Number:
Q8N5P1
Protein Gi Number:
47117585
Ncbi Gene Id Number:
84524
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.
Background Reference 1:
Lamesch, P., et al. Genomics 89(3):307-315(2007) Hwang, E.S., et al. Immunology 106(4):464-469(2002) Hwang, E.S., et al. J. Immunol. 169(1):248-253(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-863

List Size:
400 ul

List Price:

$495.00

Datasheet
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