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Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).
ZDHC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of ZDHC2 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Ramos cell line lysate.
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).

ZDHC2 Antibody

CATALOG NUMBER: 61-982

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ZDHC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the N-terminal region of human ZDHC2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZDHHC2
Additional Names:
Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Ream, Reduced expression in cancer protein, Rec, Zinc finger DHHC domain-containing protein 2, DHHC-2, Zinc finger protein 372, ZDHHC2, REAM, REC, ZNF372
Protein Accession Number:
Q9UIJ5
Protein Gi Number:
28202111
Ncbi Gene Id Number:
51201
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Palmitoyltransferase specific for GAP43 and DLG4/PSD95 (By similarity).
Background Reference 1:
Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Background Reference 2:
Sharma, C., et al. Mol. Biol. Cell 19(8):3415-3425(2008)
Background Reference 3:
Zhang, J., et al. Mol. Cell Proteomics 7(7):1378-1388(2008)
Background Reference 4:
Li, B., et al. J. Biol. Chem. 277(32):28870-28876(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-982

List Size:
400 ul

List Price:

$495.00

Datasheet
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