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Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1μg/mL.
Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 μg/mL.
Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 μg/mL.
Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 μg/mL.
Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 μg/ml.
Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1μg/mL.
Trial Size

ZEB1 Antibody

CATALOG NUMBER: 5825

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.
The immunogen is located within amino acids 530 - 580 of ZEB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of ZEB1 are known to exist.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Positive Control 3:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 124 kDa
Observed: 130 kDa
Purification:
ZEB1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZEB1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZEB1
Additional Names:
ZEB1 Antibody: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, TCF-8
Protein Accession Number:
NP_110378
Protein Gi Number:
55957123
Ncbi Gene Id Number:
6935
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.
Background Reference 1:
Funahashi J, Sekido R, Murai K, et al. Delta-crystallin enhancer protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. Dev.1993; 119:433-46.
Background Reference 2:
Postigo AA and Dean DC. Independent repressor domains in ZEB regulate muscle and T-cell differentiation. Mol. Cell Biol.1999; 19:7961-71.
Background Reference 3:
Saykally JN, Dogan S, Cleary MP, et al. The ZEB1 transcription factor is a novel repressor of adiposity in female mice. PLos One2009; 4:e8460.
Background Reference 4:
Mehta JS, Vithana EN, Tan DT, et al. Analysis of the posterior polymorphous corneal dystrophy 3 gene, TCF8, in late-onset Fuchs endothelial corneal dystrophy. Invest. Opthalmol. Vis. Sci.2008; 49:184-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5825

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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