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42-432 (0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-432 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-432 (0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ZEB2 Antibody

CATALOG NUMBER: 42-432

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-TDSRRQISNIKKEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100+38kDa bands observed in Human Liver lysates (calculated MW of 136kDa according to NP_055610.1). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour. We believe the two observed bands represent two breakdown fragments.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_055610.1; NP_001165124.1).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZEB2
Additional Names:
ZEB2, zinc finger E-box binding homeobox 2, FLJ42816, KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B, OTTHUMP00000204540, OTTHUMP00000204541, Smad-interacting protein 1, zinc finger E-box-binding homeobox 2, zinc finger homeobox 1b, zinc finger homeobox protein 1b
Protein Accession Number:
NP_055610.1,NP_001165124.1
Protein Gi Number:
7662184
Ncbi Gene Id Number:
9839

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-432

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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