ZFP112 Antibody

CATALOG NUMBER: 42-068

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-EREDDIENSHLKSYQ
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx. 48kDa band in lysates of cell line HepG2 after 2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 105kDa according to NP_037512.3. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001076804.1; NP_037512.3)
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZFP112
Additional Names:
ZFP112, zinc finger protein 112 homolog (mouse), ZNF228, zinc finger protein 228
Protein Accession Number:
NP_001076804.1,NP_037512.3
Protein Gi Number:
138175847
Ncbi Gene Id Number:
7771
Background Reference 1:
Shannon, M., Hamilton, A.T., Gordon, L., Branscomb, E. and Stubbs, L. Differential expansion of zinc-finger transcription factor loci in homologous human and mouse gene clusters. Genome Res. 13 (6A), 1097-1110 (2003).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-068

List Size:
0.1 mg

List Price:

$391.00

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