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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

ZFPM1 Antibody

CATALOG NUMBER: 25-050

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZFPM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZFPM1 antibody can be used for detection of ZFPM1 by ELISA at 1:62500. ZFPM1 antibody can be used for detection of ZFPM1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
105 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ZFPM1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ZFPM1
Additional Names:
ZFPM1, FOG, FOG1, ZNF408, ZNF89A, ZC2HC11A
Protein Accession Number:
NP_722520
Protein Gi Number:
110556644
Ncbi Gene Id Number:
161882
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZFPM1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. ZFPM1 is an essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. ZFPM1 may be involved in regulation of some genes in gonads. ZFPM1 may also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
Background Reference 1:
Sugiyama, D., (2008) Blood 111 (4), 1924-1932.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-050

List Size:
100 ul

List Price:

$519.00

Datasheet
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