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Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.
Immunocytochemistry of ZHANGFEI in K562 cells with ZHANGFEI antibody at 10 μg/mL.
Western blot analysis of ZHANGFEI in K562 cell lysate with ZHANGFEI antibody at 1 μg/mL.
Trial Size

ZHANGFEI Antibody

CATALOG NUMBER: 6191

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ZHANGFEI antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human ZHANGFEI.
The immunogen is located within amino acids 180 - 230 of ZHANGFEI.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
ZHANGFEI antibody can be used for detection of ZHANGFEI by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
ZHANGFEI Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZHANGFEI Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZHANGFEI antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CREBZF
Additional Names:
ZHANGFEI Antibody: ZF, SMILE, ZF, Host cell factor-binding transcription factor Zhangfei, HCF-binding transcription factor Zhangfei
Protein Accession Number:
NP_001034707
Protein Gi Number:
88900495
Ncbi Gene Id Number:
58487
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZHANGFEI Antibody: ZHANGFEI, also known as ZF, is a basic region-leucine zipper transcriptional factor that has been implicated in herpes virus infection cycle and related cellular processes. ZHANGFEI protein has a negatively charged active N-terminus, a leucine zipper of six heptad leucine repeats separated by a conserved six amino acid spacer, a basic domain and a bZIP region. ZHANGFEI strongly activates transcription and suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1 dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell.
Background Reference 1:
Hogan MR, Cockram GP and Lu R. Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element. FEBS Lett. 2006; 580:58-62.
Background Reference 2:
Cockram GP, Hogan MR, Burnett HF, et al. Identification and characterization of the DNA-binding properties of a Zhangfei homologue in Japanese pufferfish, Takifugu rubripes. Biochem. Biophys. Res. Commun. 2005; 339:1238-45.
Background Reference 3:
Lu R and Misra V. Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res. 2000; 28:2446-54.
Background Reference 4:
Misra V, Rapin N, Akhova O, et al. 2005. Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman. J. Biol. Chem. 2005; 280:15257-66.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6191

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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