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Western blot analysis of extracts of HL-60 cells, using ZIC3 antibody (15-140).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HL-60 cells, using ZIC3 antibody (15-140).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ZIC3 Antibody

CATALOG NUMBER: 15-140

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide of human ZIC3
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50
Positive Control 1:
HL-60
Predicted Molecular Weight:
Observed: 51kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ZIC3
Additional Names:
zinc finger protein of the cerebellum 3, zinc finger protein ZIC 3, heterotaxy 1, ZNF203, HTX1, HTX, Zic family member 3 (odd-paired homolog, Drosophila), ZIC3
Ncbi Gene Id Number:
7547
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-140

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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