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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).
ZIC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

ZIC3 Antibody

CATALOG NUMBER: 61-491

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZIC3
Additional Names:
Zinc finger protein ZIC 3, Zinc finger protein 203, Zinc finger protein of the cerebellum 3, ZIC3, ZNF203
Protein Accession Number:
O60481
Ncbi Gene Id Number:
7547
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ZIC family of C2H2-typezinc finger proteins. This nuclear protein probably functions as atranscription factor in early stages of left-right body axisformation. Mutations in this gene cause X-linked visceralheterotaxy, which includes congenital heart disease and left-rightaxis defects in organs.
Background Reference 1:
Hatayama, M., et al. Hum. Mol. Genet. 17(22):3459-3473(2008)
Background Reference 2:
Zhu, L., et al. Hum. Mutat. 29(1):99-105(2008)
Background Reference 3:
Zhu, L., et al. Hum. Mol. Genet. 16(14):1649-1660(2007)
Background Reference 4:
Chhin, B., et al. Hum. Mutat. 28(6):563-570(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-491

List Size:
400 ul

List Price:

$495.00

Datasheet
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