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46-614 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-614 (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-614 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-614 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ZIC5 Antibody

CATALOG NUMBER: 46-614

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-EIYGNPEVVRTIH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 45kDa band in human brain lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 73kDda according to NP_149123.Immunohistochemistry:Paraffin embedded Human Small Intestine, Skin and Prostate. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZIC5
Additional Names:
ZIC5, zinc finger protein of the cerebellum 5, zinc family member 5 protein, Zic family member 5 (odd-paired homolog, Drosophila)
Protein Accession Number:
NP_149123.2
Protein Gi Number:
118766337
Ncbi Gene Id Number:
85416
Background Reference 1:
Furushima K, Murata T, Matsuo I, Aizawa S. A new murine zinc finger gene, Opr. Mech Dev. 2000 Nov;98(1-2):161-4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-614

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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