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Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.
Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.
Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

ZIMP10 Antibody

CATALOG NUMBER: 6237

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (92%)
Immunogen:
ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10.
The immunogen is located within amino acids 90 - 140 of ZIMP10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
ZIMP10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIMP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIMP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZMIZ1
Additional Names:
ZIMP10 Antibody: MIZ, RAI17, ZIMP10, hZIMP10, TRAFIP10, KIAA1224, Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10
Protein Accession Number:
NP_65071
Protein Gi Number:
31543543
Ncbi Gene Id Number:
57178
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.
Background Reference 1:
Sharma M, Li X, Wang Y, et al. hZimp10 is an androgen receptor co-activator and forms a complex with sUMO-1 at replication foci. EMBO J. 2003; 22:6101-14.
Background Reference 2:
Beliakoff J and Sun Z. Zimp7 and Zimp10, two novel PIAS-like proteins, function as androgen receptor coregulators. Nucl. Recept. Signal. 2006; 4:e017.
Background Reference 3:
Lee J, Beliakoff J, and Sun Z. The novel PIAS-like protein hZimp10 is a transcriptional co-activator of the p53 tumor suppressor. Nuc. Acids Res. 2007; 35:4523-34
Background Reference 4:
Beliakoff J, Lee J, Ueno H, et al. The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. Mol. Cell. Biol. 2008; 28:282-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6237

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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