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Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.
Immunohistochemistry of ZIP5 in human spleen tissue with ZIP5 antibody at 2.5 μg/mL.
Immunofluorescence of ZIP5 in human spleen tissue with ZIP5 antibody at 20 μg/mL.
Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.
Trial Size

ZIP5 Antibody

CATALOG NUMBER: 6089

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ZIP5 antibody was raised against a 17 amino acid synthetic peptide near the center of human ZIP5.
The immunogen is located within amino acids 330 - 380 of ZIP5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
ZIP5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP5 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC39A5
Additional Names:
ZIP5 Antibody: ZIP5, LZT-Hs7, ZIP5, Zinc transporter ZIP5, Solute carrier family 39 member 5, ZIP-5
Protein Accession Number:
NP_001128667
Protein Gi Number:
206597541
Ncbi Gene Id Number:
283375
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP5 Antibody: The zinc transporter ZIP5, also known as SLC39A5, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP5 is closely related to ZIP4, and is found in many of the same tissues, including intestine, pancreas, liver, and kidney, but unlike ZIP4, ZIP5 localizes to the basolateral membrane in kidney cells and its activity is not downregulated by zinc repletion. Recent evidence suggests that ZIP4 and ZIP5 are reciprocally regulated, with translation of ZIP5 mRNA decreasing and ZIP5 protein being rapidly internalized and degraded in response to zinc deficiency.
Background Reference 1:
Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50.
Background Reference 2:
Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch.2004; 447:796-800.
Background Reference 3:
Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta.2003; 1611:16-30.
Background Reference 4:
Wang F, Kim BE, Petris MJ, et al. The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. J. Biol. Chem.2004; 279:51433-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6089

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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