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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (1 μg/mL), ZIP7, 24-029 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Brain Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Brain Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunohistochemistry Validation of ZIP7 in Human Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (6093) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of ZIP7 in Human Brain Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with 6093 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: ZIP7, 6093 (1 μg/mL), ZIP7, 24-029 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

ZIP7 Antibody

CATALOG NUMBER: 6093

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-ZIP7 antibody (6093) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7.
The immunogen is located within amino acids 20-70 of ZIP7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 4:
Cat. No. 1212 - 3T3 (Balb) Cell Lysate
Predicted Molecular Weight:
Predicted: 50kD
Observed: 50kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar ZIP7 expression profile in human cell lines detected by two independent anti-ZIP7 antibodies that recognize different epitopes, 6093 against N-terminus domain and 24-029 against recombinant fusion protein.  ZIP7 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. 

Isoforms:
Human ZIP7 has one isoform (469aa, 50kD). Mouse ZIP7 has one isoform (476aa, 51kD) and Rat ZIP7 also has one isoform (468aa, 50kD). 6093 can detect human, mouse and rat.
Purification:
ZIP7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZIP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC39A7
Additional Names:
ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4
Protein Accession Number:
NP_001070984
Protein Gi Number:
117553619
Ncbi Gene Id Number:
7922
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZIP7 Antibody: The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.
Background Reference 1:
Dufner-Beattie et al. J. Biol. Chem.2003; 278:50142-50.
Background Reference 2:
Eide. Pflugers Arch.2004; 447:796-800.
Background Reference 3:
Taylor and Nicohlson. Biochim. Biophys. Acta.2003; 1611:16-30.
Background Reference 4:
Lai et al. Genomics1994; 23:338-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6093

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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