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Antibody is used in Western blot to detect ZMPSTE24 in T-47D cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect ZMPSTE24 in T-47D cell lysate.

ZMPSTE24 Antibody

CATALOG NUMBER: 61-386

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ZMPSTE24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-470 amino acids from the C-terminal region of human ZMPSTE24.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZMPSTE24
Additional Names:
CAAX prenyl protease 1 homolog, Farnesylated proteins-converting enzyme 1, FACE-1, Prenyl protein-specific endoprotease 1, Zinc metalloproteinase Ste24 homolog, ZMPSTE24, FACE1, STE24
Protein Accession Number:
O75844
Ncbi Gene Id Number:
10269
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZMPSTE24 is a zinc metalloprotease similar to yeast Ste24p. It is an integral membrane protein belonging to peptidase family M48 and is found in the endoplasmic reticulum and possibly in the Golgi compartment. It is thought to be involved in the proteolytic processing of farnesylated proteins.
Background Reference 1:
Freije, J.M., et al., Genomics 58(3):270-280 (1999).
Background Reference 2:
Kumagai, H., et al., Biochim. Biophys. Acta 1426(3):468-474 (1999).
Background Reference 3:
Tam, A., et al., J. Cell Biol. 142(3):635-649 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-386

List Size:
400 ul

List Price:

$495.00

Datasheet
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