Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of ZMYM2 in EL4 cell lysate with ZMYM2 antibody at (A) 0.125 and (B) 0.25 μg/mL.
Immunocytochemistry of ZMYM2 in HeLa cells with ZMYM2 antibody at 10 μg/mL.
Immunofluorescence of ZMYM2 in HeLa cells with ZMYM2 antibody at 20 μg/mL.
Western blot analysis of ZMYM2 in EL4 cell lysate with ZMYM2 antibody at (A) 0.125 and (B) 0.25 μg/mL.
Trial Size

ZMYM2 Antibody

CATALOG NUMBER: 6135

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZMYM2 antibody was raised against a 17 amino acid peptide near the amino terminus of human ZMYM2.
The immunogen is located within amino acids 120 - 170 of ZMYM2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ZMYM2 antibody can be used for detection of ZMYM2 by Western blot at 0. 125 - 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of ZMYM2 are known to exist; this antibody will detect only the longest isoform. ZMYM2 antibody is predicted to not cross-react with other ZMYM protein family members.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
ZMYM2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZMYM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZMYM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZMYM2
Additional Names:
ZMYM2 Antibody: FIM, MYM, RAMP, SCLL, ZNF198, FIM, Zinc finger MYM-type protein 2, Fused in myeloproliferative disorders protein
Protein Accession Number:
CAH71822
Protein Gi Number:
300192961
Ncbi Gene Id Number:
7750
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Zinc-finger proteins contain DNA-binding domains characterized by the unique role of zinc and have a wide variety of functions such as transcriptional activation or repression (1). The protein folding and the DNA binding ability are governed by the coordination of a zinc ion (1-3). As a member of the MYM (myeloproliferative and mental retardation) gene family, ZMYM2 has been shown to associate with and stabilize the LSD1-CoREST-HDAC1 (LCH) complex of chromatin through its MYM-type zinc fingers, thereby enhancing the transcriptional repression of several genes (4). Translocation of this gene with the fibroblast growth factor receptor-1 gene (FGFR1) results in a fusion gene, which may be a cause of stem cell leukemia lymphoma syndrome (SCLL) (5).
Background Reference 1:
Rosenfeld R and Margalit H. Zinc fingers: conserved properties that can distinguish between spurious and actual DNA-binding motifs. J. Biomol. Struct. Dyn. 1993; 11:557-70.
Background Reference 2:
Gregory SG, Barlow KF, McLay KE, et al. The DNA sequence and biological annotation of human chromosome 1. Nature 2006; 441:315-21.
Background Reference 3:
Sohal J, Reiter A, Goldman JM, et al. Cloning of ZNF237, a novel member of the MYM gene family that maps to human chromosome 13q11→q12. Cytogenet. Cell Genet. 2000; 89:24-8
Background Reference 4:
Gocke CB and Yu H. ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers. PLoS One 2008; 3:e3255

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6135

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369