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Western blot analysis in CEM cell line lysates (35ug/lane).
ZMYND10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

ZMYND10 Antibody

CATALOG NUMBER: 56-227

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZMYND10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-348 amino acids from the Central region of human ZMYND10.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZMYND10
Additional Names:
Zinc finger MYND domain-containing protein 10, Protein BLu, ZMYND10, BLU
Protein Accession Number:
O75800
Ncbi Gene Id Number:
51364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BLU is a candidate tumor suppressor gene, that spans 4.5 kb on 3p21.3. It encodes a 50 kd protein, which is commonly found in transcription repressors. It is suspected that BLU has a function in cell cycle progression. BLU is a stress-responsive gene regulated by E2F.12 It is commonly found to be downregulated in non-small cell lung cancer, esophagus squamous cell carcinoma and nasopharyngeal carcinoma (NPC).
Background Reference 1:
Shao, Y., et al. Cancer Invest. 28(6):642-648(2010)
Background Reference 2:
Lorente, A., et al. Brain Pathol. 19(2):279-292(2009)
Background Reference 3:
Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)
Background Reference 4:
Marsit, C.J., et al. Int. J. Cancer 114(2):219-223(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-227

List Size:
400 ul

List Price:

$495.00

Datasheet
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