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Western blot analysis in U251 cell line lysates (35ug/lane).
ZNF197 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).

ZNF197 Antibody

CATALOG NUMBER: 55-247

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZNF197 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the N-terminal region of human ZNF197.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
119 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF197
Additional Names:
Zinc finger protein 197, Zinc finger protein with KRAB and SCAN domains 9, ZnF20, pVHL-associated KRAB domain-containing protein, ZNF197, ZKSCAN9, ZNF166
Protein Accession Number:
O14709
Ncbi Gene Id Number:
10168
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF197 product belongs to the zinc finger proteinsuperfamily, members of which are regulatory proteins characterizedby nucleic acid-binding zinc finger domains. The encoded proteincontains 20 tandemly arrayed C2H2-type zinc fingers, aKruppel-associated box (KRAB) domain, and a SCAN box. Thistranscript turns over rapidly and contains 3' UTR AUUUA motifs,which are often a hallmark of rapid turnover. It is overexpressedin some thyroid papillary carcinomas. ZNF197 is located in acluster of zinc finger genes at 3p21. Two alternatively splicedtranscripts encoding different isoforms have been described.
Background Reference 1:
Li, Z., et al. EMBO J. 22(8):1857-1867(2003)
Background Reference 2:
Gonsky, R., et al. Nucleic Acids Res. 25(19):3823-3831(1997)
Background Reference 3:
Calabro, V., et al. Hum. Genet. 95(1):18-21(1995)
Background Reference 4:
Pengue, G., et al. Hum. Mol. Genet. 2(6):791-796(1993)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-247

List Size:
400 ul

List Price:

$495.00

Datasheet
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