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Western blot analysis of ZNF202 Antibody in HepG2 cell line lysates (35ug/lane)
ZNF202 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ZNF202 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Fluorescent confocal image of MDA-MB231 cell stained with ZNF202 Antibody . MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF202 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ZNF202 immunoreactivity is localized to nucleus significantly.
Western blot analysis of ZNF202 Antibody in HepG2 cell line lysates (35ug/lane)

ZNF202 Antibody

CATALOG NUMBER: 64-108

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZNF202 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the Central region of human ZNF202.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF202
Additional Names:
Zinc finger protein 202, Zinc finger protein with KRAB and SCAN domains 10, ZNF202, ZKSCAN10
Protein Accession Number:
O95125
Ncbi Gene Id Number:
7753
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF202 (Zinc finger protein 202) is a transcriptional repressor of genes affecting the vascular endothelium as well as lipid metabolism and energy homeostasis. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
Background Reference 1:
Aberg, K., et al. Biol. Psychiatry 67(3):279-282(2010)
Background Reference 2:
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
Background Reference 3:
Patterson, E.S., et al. Physiol. Genomics 34(3):277-284(2008)
Background Reference 4:
Stene, M.C., et al. J. Am. Coll. Cardiol. 52(5):369-377(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-108

List Size:
400 ul

List Price:

$495.00

Datasheet
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