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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

ZNF446 Antibody

CATALOG NUMBER: 29-042

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF446.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZNF446 antibody can be used for detection of ZNF446 by ELISA at 1:1562500. ZNF446 antibody can be used for detection of ZNF446 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
49 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ZNF446 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ZNF446
Additional Names:
ZNF446, ZSCAN30, ZSCAN52, ZKSCAN20
Protein Accession Number:
NP_060378
Protein Gi Number:
8923582
Ncbi Gene Id Number:
55663
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF446 contains a KRAB and three C (2)H (2) zinc fingers. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, suggesting that the ZNF446 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.
Background Reference 1:
Liu, F., et al., (2005) Biochem. Biophys. Res. Commun. 333 (1), 5-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-042

List Size:
100 ul

List Price:

$473.00

Datasheet
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