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Immunohistochemistry of ZNF521 in human kidney tissue with ZNF521 antibody at 5 μg/mL.
Immunohistochemistry of ZNF521 in human kidney tissue with ZNF521 antibody at 5 μg/mL.
Trial Size

ZNF521 Antibody

CATALOG NUMBER: 6065

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
ZNF521 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZNF521.
The immunogen is located within amino acids 1160 - 1210 of ZNF521.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P
Application Note:
ZNF521 antibody can be used for detection of ZNF521 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
ZNF521 antibody is human specific.
Positive Control 1:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
ZNF521 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZNF521 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF521
Additional Names:
ZNF521 Antibody: EHZF, Evi3, EHZF, LIP3, Zinc finger protein 521, Early hematopoietic zinc finger protein
Protein Accession Number:
NP_056276
Protein Gi Number:
24308069
Ncbi Gene Id Number:
25925
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
Background Reference 1:
Warming S, Liu P, Suzuki T, et al. Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes a EBFAZ-related Kruppel-like zinc finger protein. Blood 2003; 101:1934-40.
Background Reference 2:
Matsubara E, Sakai I, Yamanouchi J, et al. The role of zinc finger protein 521/early hematopoietic zinc finger protein in erythroid cell differentiation. J. Biol. Chem. 2009; 284:3480-7.
Background Reference 3:
Wu M, Hesse E, Morvan F, et al. Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone 2009; 44:528-36.
Background Reference 4:
Hesse E, Saito H, Kiviranta R, et al. Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. J. Cell Biol. 2010; 191:1271-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6065

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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