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Western blot analysis of ZNF536 in human brain tissue lysate with ZNF536 antibody at 1 μg/mL.
Immunohistochemistry of ZNF536 in human brain tissue with ZNF536 antibody at 5 μg/mL.
Immunofluorescence of ZNF536 in human brain tissue with ZNF536 antibody at 20 μg/mL.
Western blot analysis of ZNF536 in human brain tissue lysate with ZNF536 antibody at 1 μg/mL.
Trial Size

ZNF536 Antibody

CATALOG NUMBER: 5501

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ZNF536 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536.
The immunogen is located within amino acids 30 - 80 of ZNF536.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ZNF536 antibody can be used for detection of ZNF536 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
ZNF536 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZNF536 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZNF536 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF536
Additional Names:
ZNF536 Antibody: KIAA0390, Zinc finger protein 536
Protein Accession Number:
NP_055532.1
Protein Gi Number:
7662092
Ncbi Gene Id Number:
9745
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZNF536 Antibody: ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.
Background Reference 1:
Qin Z, Ren F, Xu X, et al. ZNF536, a novel zinc finger protein specifically expressed in the brain, negatively regulates neuron differentiation by repressing retinoic acid-induced gene transcription. Mol. Cell. Biol.2009; 29:3633-43.
Background Reference 2:
Sakai T, Hino K, Wada S, et al. Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor. DNA Res.2003; 10:155-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5501

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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