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Western blot analysis of ZNF821 in Jurkat cell lysate with ZNF821 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ZNF821 in Jurkat cells with ZNF821 antibody at 2.5 μg/mL.
Immunofluorescence ofZNF821 in Jurkat cells with ZNF821 antibody at 20 μg/mL.
Western blot analysis of ZNF821 in Jurkat cell lysate with ZNF821 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZNF821 Antibody

CATALOG NUMBER: 7537

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
ZNF821 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ZNF821.
The immunogen is located within amino acids 270 - 320 of ZNF821.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ZNF821 antibody can be used for detection of ZNF821 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ZNF821 antibody is human and mouse reactive. At least three isoforms of ZNF821 are known to exist; this antibody will detect the two largest isoforms.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Predicted: 41 kDa
Observed: 38 kDa
Purification:
ZNF821 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZNF821 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZNF821 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ZNF821
Additional Names:
ZNF821 Antibody: Zinc finger protein 821
Protein Accession Number:
NP_001188482.1
Protein Gi Number:
320118856
Ncbi Gene Id Number:
55565
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ZNF821 protein contains two C2H2 zinc finger motifs and a score-and-three (23)-amino acid peptide repeat (STPR) domain. The STPR domain of the encoded protein binds to double stranded DNA and may also contain a nuclear localization signal, suggesting that this protein interacts with chromosomal DNA (1). The exact function of ZNF821, however, remains unknown.
Background Reference 1:
Nonaka Y, Muto H, Aizawa T, et al. STPR, a 23-amino acid tandem repeat domain, found in the human function-unknown protein ZNF821. Biochemistry 2010; 49:8367-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7537

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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