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42-230 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-230 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction staining. The nuclear stain is DAPI
42-230 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ZO-1 Antibody

CATALOG NUMBER: 42-230

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PKPTSQNQFSEHDKT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cell junctions of A431 cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)
Positive Control 1:
Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TJP1
Additional Names:
TJP1, tight junction protein 1 (zona occludens 1), DKFZp686M05161, MGC133289, ZO-1, OTTHUMP00000174520, tight junction protein 1, tight junction protein ZO-1, zona occludens 1, zonula occludens 1 protein
Protein Accession Number:
NP_003248.3,NP_783297.2
Protein Gi Number:
116875767
Ncbi Gene Id Number:
7082
Background Reference 1:
Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O1Banion MK, Stojanovic K, Sagare A, Boillee S, Cleveland DW, Zlokovic BV, ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat. Neurosci. 2008 Apr 11 (4): 420-2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-230

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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