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Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)
ZO1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)

ZO1 Antibody

CATALOG NUMBER: 61-661

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1570-1600 amino acids from the C-terminal region of human ZO1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
195 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TJP1
Additional Names:
Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1
Protein Accession Number:
Q07157
Protein Gi Number:
85700443
Ncbi Gene Id Number:
7082
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions.
Background Reference 1:
Ohira, M., et al. Int. J. Mol. Med. 24(6):829-835(2009)
Background Reference 2:
Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)
Background Reference 3:
Meerschaert, K., et al. Cell. Mol. Life Sci. 66(24):3951-3966(2009)
Background Reference 4:
Kirschner, N., et al. Am. J. Pathol. 175(3):1095-1106(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-661

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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