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Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
ZP4 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy for immunohistochemistry.
Western Blot at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.

ZP4 Antibody

CATALOG NUMBER: 56-605

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZP4
Additional Names:
Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
Protein Accession Number:
Q12836
Protein Gi Number:
46397080
Ncbi Gene Id Number:
57829
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The zona pellucida is an extracellular matrix thatsurrounds the oocyte and early embryo. It is composed primarily ofthree or four glycoproteins with various functions duringfertilization and preimplantation development. The nascent proteincontains a N-terminal signal peptide sequence, a conserved ZPdomain, a consensus furin cleavage site, and a C-terminaltransmembrane domain. It is hypothesized that furin cleavageresults in release of the mature protein from the plasma membranefor subsequent incorporation into the zona pellucida matrix.However, the requirement for furin cleavage in this process remainscontroversial based on mouse studies. Previously, this gene hasbeen referred to as ZP1 or ZPB and thought to have similarfunctions as mouse Zp1. However, a human gene with highersimilarity and chromosomal synteny to mouse Zp1 has been assignedthe symbol ZP1 and this gene has been assigned the symbol ZP4.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
Background Reference 3:
Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
Background Reference 4:
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-605

List Size:
400 ul

List Price:

$495.00

Datasheet
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