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Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in WB on Human HeLa cells at 1:200.
Antibody used in WB on Human Jurkat 1.25 ug/ml.

ZYX Antibody

CATALOG NUMBER: 28-075

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZYX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ZYX antibody can be used for detection of ZYX by ELISA at 1:62500. ZYX antibody can be used for detection of ZYX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
61 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ZYX antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ZYX
Additional Names:
ZYX, ESP-2, HED-2
Protein Accession Number:
NP_003452
Protein Gi Number:
4508047
Ncbi Gene Id Number:
7791
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
Background Reference 1:
Li, B., et al., (2004) J. Biol. Chem. 279 (19), 20401-20410.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-075

List Size:
100 ul

List Price:

$473.00

Datasheet
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