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42-257 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-257 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ZYX Antibody

CATALOG NUMBER: 42-257

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QQREKPRVQEKQH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kDa according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Specificity:
Reported variants represent identical protein: NP_003452.1, NP_001010972.1
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ZYX
Additional Names:
ZYX, zyxin, ESP-2, HED-2
Protein Accession Number:
NP_003452.1
Protein Gi Number:
4508047
Ncbi Gene Id Number:
7791
Background Reference 1:
Li B, Zhuang L, Trueb B, Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. The Journal of biological chemistry 2004 May 279 (19): 20401-10.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-257

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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