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43-456 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-456 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
43-456 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

proteinase 3 / myeloblastin Antibody

CATALOG NUMBER: 43-456

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HNVRTQEPTQQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1ug/ml. Primary incubation was 1 hour
Predicted Molecular Weight:
Approx 27kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
proteinase 3 / myeloblastin
Additional Names:
PRTN3, proteinase 3, ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP4, P29, PR-3, PR3, C-ANCA antigen, NP-4, Wegener granulomatosis autoantigen, azurophil granule protein 7, leukocyte proteinase 3, myeloblastin, neutrophil proteinase 4, serine proteinase, neutrophil, wegener autoantigen
Protein Accession Number:
NP_002768.3
Protein Gi Number:
71361688
Ncbi Gene Id Number:
5657
Background Reference 1:
Bae S, Kang T, Hong J, Lee S, Choi J, Jhun H, Kwak A, Hong K, Kim E, Jo S, Kim S.Contradictory functions (activation/termination) of neutrophil proteinase 3 enzyme (PR3) in interleukin-33 biological activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-456

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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