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43-692 (0.3ug/ml) staining of HEK293 (A) and HeLa (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-692 (0.3ug/ml) staining of HEK293 (A) and HeLa (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RE1-silencing transcription factor Antibody

CATALOG NUMBER: 43-692

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PETPGAEDKGKSSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 140kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Specificity:
Reported variants represent identical protein: NP_001180437.1, NP_005603.3
Predicted Molecular Weight:
Approx 140kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RE1-silencing transcription factor
Additional Names:
REST, RE1-silencing transcription factor, NRSF, XBR, RE1-silencing transcription factor variant E1a/E2/E3/E4c, RE1-silencing transcription factor variant E1a/E2/E3/E5, RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4, RE1-silencing transcription factor variant E1a/E2/E4, RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j, RE1-silencing transcription factor variant E1b/E2/E3/E5, RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i, RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4, RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4, RE1-silencing transcription factor variant E1c/E2/E3/E5, RE1-silencing transcription factor variant E1c/E2g/E3/E4, neural-restrictive silencer factor, neuron restrictive silencer factor, repressor binding to the X2 box
Protein Accession Number:
NP_005603.3
Protein Gi Number:
301897977
Ncbi Gene Id Number:
5978
Background Reference 1:
Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, Yang TH, Kim HM, Drake D, Liu XS, Bennett DA, Colaiácovo MP, Yankner BA.REST and stress resistance in ageing and Alzheimer's disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-692

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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