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Flow cytometric analysis of HepG2 cells using REG3A Antibody (green) compared to an isotype control of rabbit IgG(blue). AP20597c was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody at 1:1000.
Flow cytometric analysis of HepG2 cells using REG3A Antibody (green) compared to an isotype control of rabbit IgG(blue). AP20597c was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

REG3A Antibody

CATALOG NUMBER: 61-054

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This REG3A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 129-163 amino acids from the C-terminal region of human REG3A.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
REG3A
Additional Names:
Regenerating islet-derived protein 3-alpha, REG-3-alpha, Hepatointestinal pancreatic protein, HIP/PAP, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, Reg III-alpha, Regenerating islet-derived protein 3-alpha 165 kDa form, Regenerating islet-derived protein 3-alpha 15 kDa form, REG3A, HIP, PAP, PAP1
Protein Accession Number:
Q06141
Protein Gi Number:
464341
Ncbi Gene Id Number:
5068
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Background Reference 1:
Itoh T.,et al.Biochim. Biophys. Acta 1172:184-186(1993).
Background Reference 2:
Orelle B.,et al.J. Clin. Invest. 90:2284-2291(1992).
Background Reference 3:
Lasserre C.,et al.Cancer Res. 52:5089-5095(1992).
Background Reference 4:
Dusetti N.J.,et al.Genomics 19:108-114(1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-054

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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