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43-362 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-362 (0.5ug/ml) staining of NIH3T3 (A), 3T3L1 (B), Mouse Lymph Node (C) and NSO (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-362 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RPA1 / RPA70 Antibody

CATALOG NUMBER: 43-362

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: CKSYEDATKITVRSN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of Mouse Lymph Node. Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 70 + 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RPA1 / RPA70
Additional Names:
RPA1, replication protein A1, 70kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein
Protein Accession Number:
NP_002936.1
Protein Gi Number:
4506583
Ncbi Gene Id Number:
6117
Background Reference 1:
Lada AG, Waisertreiger IS, Grabow CE, Prakash A, Borgstahl GE, Rogozin IB, Pavlov YI.Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-362

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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