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Western blot analysis in K562 cell line lysates (35ug/lane).
RPS4Y1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

RPS4Y1 Antibody

CATALOG NUMBER: 56-655

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Xenopus
Immunogen:
This RPS4Y1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human RPS4Y1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS4Y1
Additional Names:
40S ribosomal protein S4, Y isoform 1, RPS4Y1, RPS4Y
Protein Accession Number:
P22090
Protein Gi Number:
133948
Ncbi Gene Id Number:
6192
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cytoplasmic ribosomes, organelles that catalyze proteinsynthesis, consist of a small 40S subunit and a large 60S subunit.Together these subunits are composed of 4 RNA species andapproximately 80 structurally distinct proteins. This gene encodesribosomal protein S4, a component of the 40S subunit. Ribosomalprotein S4 is the only ribosomal protein known to be encoded bymore than one gene, namely this gene and ribosomal protein S4,X-linked (RPS4X). The 2 isoforms encoded by these genes are notidentical, but are functionally equivalent. Ribosomal protein S4belongs to the S4E family of ribosomal proteins. It has beensuggested that haploinsufficiency of the ribosomal protein S4 genesplays a role in Turner syndrome; however, this hypothesis iscontroversial. As is typical for genes encoding ribosomal proteins,there are multiple processed pseudogenes of this gene dispersedthrough the genome.
Background Reference 1:
Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004)
Background Reference 2:
Vawter, M.P., et al. Neuropsychopharmacology 29(2):373-384(2004)
Background Reference 3:
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Background Reference 4:
Skaletsky, H., et al. Nature 423(6942):825-837(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-655

List Size:
400 ul

List Price:

$495.00

Datasheet
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