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Western blot analysis of SATB1 in SK-N-SH cell lysate with SATB1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SATB1 in human brain tissue with SATB1 antibody at 2.5 μg/mL.
Immunofluorescence of SATB1(NT) in Human Brain cells with SATB1(NT) antibody at 20 μg/mL.
Western blot analysis of SATB1 in SK-N-SH cell lysate with SATB1 antibody at (A) 1 and (B) 2 μg/mL.
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SATB1 Antibody

CATALOG NUMBER: 4679

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SATB1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SATB1.
The immunogen is located within the first 50 amino acids of SATB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SATB1 antibody can be used for detection of SATB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SATB1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
SATB1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SATB1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SATB1
Additional Names:
SATB1 Antibody: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
Protein Accession Number:
Q01826
Protein Gi Number:
417747
Ncbi Gene Id Number:
6304
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SATB1 Antibody: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
Background Reference 1:
Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol.1995; 15:456-65.
Background Reference 2:
Szemes M, Gyorgy A, Pawaletz C, et al. Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain. Neurochem. Res.2006; 31:237-46.
Background Reference 3:
Purbey PK, Singh S, Kumar PP, et al. PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB1. Nucleic Acids Res.2008; 36:2107-22.
Background Reference 4:
Cai S, Han HJ and Kohwi-Shigematsu T. Tissue-specific nuclear architecture and gene expression regulated by SATB1. Nat. Genet.2003; 34:42-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4679

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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