Monoclonal Antibody Development

Antigen Purity

• For monoclonal antibodies, >90% antigen purity is the standard to ensure specificity

Protein Antigen

• Quantity: 1mg with no tag at concentrations of 1 mg/ml or higher required for immunization plus 1-2 mg required for screening
• Size: at least 10 kDa
• Production Method: Use of native protein is preferred over recombinant protein. If supplying a recombinant protein, use an expression system with a similar origin as your protein target (for example, use a mammalian expression system for human proteins) to maximize probability of proper protein conformation and post-translational modifications and increase likelihood of developing an antibody that will recognize the native protein.
• Tags: No tags for immunization and large fusion proteins such as GST or MBP must be cleaved to maximize immune response against the target of interest. Tags, however, may be acceptable for screening; contact one of our custom antibody experts to discuss your project today.

Peptide Antigen

• Quantity
  • 5 mg for us to conjugate, or
  • 1 mg conjugated to a carrier protein (KLH preferred), and
  • 2-3 mg unconjugated for screening
• Antigens in Solution: Antigens should be submitted at a concentration of 1 mg/ml or higher. Please specify the buffer, concentration, total volume, and total amount. Buffers such as PBS, Tris, <4M urea are preferred; SDS and citrate buffers are also acceptable. Please note for services such as ELISA and immuno-affinity purification the antigen needs to be soluble. Please submit an antigen submission form with the information above prior to sending your antigen.