Monoclonal antibodies have higher specificity and affinity compared to polyclonal antibodies. ProSci uses hybridoma development for mouse monoclonal antibody development, which is a more established technolo
Recombinant rabbit monoclonal antibodies are excellent tools for challenging targets such as small molecules or post-translational modifications. They have high affinity due to the rabbit’s robust immune system. Several methods are used to develop custom rabbit monoclonal antibodies, such as hybridoma generation, phage display, or B-cell cloning. ProSci’s B-cell development platform allows for careful selection of the ideal antibodies and preservation and immortalization through conversion to a recombinant. Recombinant antibodies provide the specificity of a monoclonal antibody but, more importantly, eliminate the potential loss of a hybridoma clone through preservation of the heavy and light chain sequences which minimizes lot-to-lot production variability through production as recombinant proteins.
Rabbits can provide benefits over mice when developing monoclonal antibodies. Rabbit spleens contain more B cells, increasing the probability of finding the right clone for your needs. Rabbits have a larger CDR-3 repertoire which can offer better selectivity and specificity to a desired antigen due to the higher incidence of longer CDR-3 loops in rabbits when compared to mice.
Longer CDR-3s can improve affinities to targets by 10 to 100-fold over murine antibodies. With a unique immune system that reduces immunodominance, they are the ideal host to develop antibodies against a wider range of epitopes and are preferred for developing antibodies against small molecules.
Outsourcing recombinant rabbit monoclonal antibody development to ProSci’s antibody experts allows you to focus on your core research.
gy compared to recombinant antibody methods.
We have over 25 years of experience developing monoclonal antibodies; working with customers worldwide across a broad range of research areas in academia and industry to take on challenging antibody needs. ProSci antibody experts allow researchers to focus on optimizing other aspects of their research.
ProSci’s recombinant rabbit monoclonal antibody development platform sorts and isolates antigen-specific B-cells, preserving native heavy and light chain pairs. The preservation of native pairs minimizes the amount of screening and sequence bias found in phage display development of conventional antibodies. It eliminates the chromosomal recombination found during fusion to generate a hybridoma. The antibody-secreting B-cells are cultured individually and screened after two weeks for antibodies. Heavy and light chains from the desired B-cells are then cloned and transiently transfected. The resulting supernatant is screened again to ensure the selection of antibodies that meet our clients’ needs.
| Hybridoma | Phage Display | Single B Cell Cloning | |
|---|---|---|---|
| Starting Material | B Cells | B Cells | B Cells |
| Antibody Diversity | Medium | Low | High |
Phase
Procedure
Detailed Procedure
I
Immunization
II
Single-cell Sorting, Culturing, and Screening
III
Antibody Cloning and Small-Scale Expression
IV
Sequencing, Expression, and Purification
Antigen Purity
Protein Antigen Specifications
Protein Antigens in Solution
Please submit an antigen submission form with the information above prior to sending your antigen.
Peptide Antigen Specifications
Other Antigen Types
Protein antigens are preferred for rabbit monoclonal antibody development; for other antigen types refer to our antigen requirements guide or contact us.
Rabbit monoclonal antibody development using client-provided splenocytes
Rabbit monoclonal antibody development
Additional Small-Scale Purified Antibody If additional purified antibodies are needed, we offer purified antibodies from 30 ml culture for additional available clones during the selection process.
Additional Final Clones If you want more final clones than the 5 offered in the packages, we offer additional clones, including the antibody sequence and DNA.
Customized Sorting and Screening If you need additional controls during screening, contact our antibody experts to design the optimal screening and sorting strategies to develop the desired antibodies.
Because of the nature of developing antibodies from immunization to final clones, we implement project milestones consisting of clear, actionable go/no-go decision points during the development process. Our experts review the antibody development progress at each milestone to ensure your antibody success.
Once antibody development is complete, production can start. We offer transient expression of rabbit monoclonal antibodies in a mammalian expression system to produce larger quantities of purified rabbit monoclonal antibodies.
ProSci’s team of antibody specialists will help accelerate your research, therapeutic, or diagnostic project from idea to reality. Take advantage of decades of experience and contact our Team today for a free consultation on your project.
