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42-279 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-279 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-279 (5ug/ml) staining of formaldehyde-fixed SH5Y5Y and detected with Alexa 488 in confocal microscopy. Data obtained from Dr. Schallburg Nielsen, Aarhus University Denmark.
42-279 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SNX1 Antibody

CATALOG NUMBER: 42-279

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TNSSKPQPTYEELE
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 75kDa band observed in Human lysates of cell line HeLa (calculated MW of 59.1kDa according to NP_003090.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5ug/ml.Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SNX1
Additional Names:
SNX1, sorting nexin 1, HsT17379, MGC8664, SNX1A, Vps5, sorting nexin 1A
Protein Accession Number:
NP_003090.2,NP_690039.1
Protein Gi Number:
23111034
Ncbi Gene Id Number:
6642
Background Reference 1:
Mari M, Bujny MV, Zeuschner D, Geerts WJ, Griffith J, Petersen CM, Cullen PJ, Klumperman J, Geuze HJ, SNX1 defines an early endosomal recycling exit for sortilin and mannose 6-phosphate receptors. Traffic (Copenhagen, Denmark) 2008 Mar 9 (3): 380-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-279

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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