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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ST6GALNAC1 Antibody

CATALOG NUMBER: 56-398

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ST6GALNAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human ST6GALNAC1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ST6GALNAC1
Additional Names:
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1, GalNAc alpha-2,6-sialyltransferase I, ST6GalNAc I, ST6GalNAcI, Sialyltransferase 7A, SIAT7-A, ST6GALNAC1, SIAT7A
Protein Accession Number:
Q9NSC7
Protein Gi Number:
21759444
Ncbi Gene Id Number:
55808
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glycosylation of proteins affects cell-cell interaction,interactions with the matrix, and the functions of intracellularmolecules. ST6GALNAC1 transfers a sialic acid, N-acetylneuraminicacid (NeuAc), in an alpha-2,6 linkage to O-linked GalNAc residues.The cancer-associated sialyl-Tn (sTn) antigen is formed byST6GALNAC1-catalyzed sialylation of GalNAc residues on mucins(Ikehara et al., 1999 [PubMed 10536037]; Sewell et al., 2006[PubMed 16319059]).
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Hassinen, A., et al. J. Biol. Chem. 285(23):17771-17777(2010)
Background Reference 3:
Patani, N., et al. Cancer Genomics Proteomics 5(6):333-340(2008)
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-398

List Size:
400 ul

List Price:

$495.00

Datasheet
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