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46-448 (0.003ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-448 (0.003ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

STX1A Antibody

CATALOG NUMBER: 46-448

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: KDRTQELRTAKD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Mouse Brain lysates (calculated MW of 33.0kDa according to NP_004594.1 and 33.2kDa according to NP_443106.1). Recommended concentration: 0.01-0.03ug/ml.
Specificity:
Please note this product is expected to recognize the products of 2 different genes. STX1A (NP_004594.1and NP_001159375.1) and STX1B (NP_443106.1).
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
STX1A / STX1B
Additional Names:
STX1A, syntaxin 1A (brain) , HPC-1, STX1, p35-1, SYN1A, OTTHUMP00000174615, OTTHUMP00000174616, OTTHUMP00000174617, OTTHUMP00000174618, neuron-specific antigen HPC-1, syntaxin-1A, STX1B, syntaxin 1B, STX1B1, STX1B2, FP17469, OTTHUMP00000163224, syntaxin 1B1, syntaxin 1B2, syntaxin-1B, syntaxin-1B1, syntaxin-1B2
Protein Accession Number:
NP_004594.1,NP_001159375.1,NP_443106.1
Protein Gi Number:
4759182
Ncbi Gene Id Number:
6804;112755
Background Reference 1:
Kang Y, Leung YM, Manning-Fox JE, Xia F, Xie H, Sheu L, Tsushima RG, Light PE, Gaisano HY. Syntaxin-1A inhibits cardiac KATP channels by its actions on nucleotide binding folds 1 and 2 of sulfonylurea receptor 2A. J Biol Chem. 2004 Nov 5;279(45):47125-31. Epub 2004 Aug 31.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-448

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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