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43-288 (0.03ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-288 (0.03ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TNNI2 Antibody

CATALOG NUMBER: 43-288

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: KRNRAITARRQHLKS-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 21.3kDa according to NP_003273.1). Recommended concentration: 0.03-0.1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Predicted Molecular Weight:
Approx 25kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TNNI2
Additional Names:
TNNI2, troponin I type 2 (skeletal, fast), AMCD2B, DA2B, FSSV, fsTnI, fast-twitch skeletal muscle troponin I, troponin I fast twitch 2, troponin I, fast skeletal muscle, troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform, troponin I, skeletal, fast
Protein Accession Number:
NP_003273.1,NP_001139313.1
Protein Gi Number:
4507621
Ncbi Gene Id Number:
7136
Background Reference 1:
Moran CM, Garriock RJ, Miller MK, Heimark RL, Gregorio CC, Krieg PA.Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-288

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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