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46-567 (0.3ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-567 (0.3ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VANGL2 Antibody

CATALOG NUMBER: 46-567

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-QKYLRTTKQQPYH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Brain lysates (calculated MW of 59.7kDa according to NP_065068.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human Lung and Ovary for this particular batch.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VANGL2
Additional Names:
VANGL2, vang-like 2 (van gogh, Drosophila) , KIAA1215, LPP1, LTAP, MGC119403, MGC119404, STBM, homolog of Drosophila strabismus, loop-tail-associated protein, vang (van gogh, Drosophila)-like 2, vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (D
Protein Accession Number:
NP_065068.1
Protein Gi Number:
62955805
Ncbi Gene Id Number:
57216
Background Reference 1:
Kibar Z, Vogan KJ, Groulx N, Justice MJ, Underhill DA, Gros P. Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail. Nat Genet. 2001 Jul;28(3):251-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-567

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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