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Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.

VE-Cadherin (phospho Tyr731) Antibody

CATALOG NUMBER: 80-000

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
VE-Cadherin (phospho Tyr731) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine731 (H-I-Y (p)-G-Y) derived from Human VE-Cadherin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731.
Predicted Molecular Weight:
130 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDH5
Additional Names:
Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CDH5, CD144 antigen
Protein Accession Number:
NP_001786.2
Protein Gi Number:
166362713
Ncbi Gene Id Number:
1003
Background:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen.
Background Reference 1:
Wessel F, et al. (2014) Nat Immunol 15, 223-30
Background Reference 2:
Sawada J, et al. (2012) Cancer Cell 22, 235-49
Background Reference 3:
Turowski P, et al. (2008) J Cell Sci 121, 29-37

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-000

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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