Premier Client-Provided Antigen Time: ~18-25 weeks 12-16 weeks Phase I: Immunization 3 BALB/c mice 1 Pre-immune serum collection 5 Immunizations 4 Serum collections ELISA testing of serum 2-3 weeks Phase II: Fusion Fusion of 1 mouse spleen ELISA screening 4-6 weeks Phase III: Subcloning 2 subcloning steps Final clone isotyping Deliverables ELISA data Supernatant for […]
Premier Client-Provided Splenocytes Time: ~7-12 weeks 2-3 weeks Phase II: Single-Cell Sorting, Culturing, and Screening Splenocyte isolation from 1 rabbit Sorting and culturing antibody secreting B-cells ELISA screening 4-8 weeks Phase III: Antibody Cloning and Small-Scale Expression Cloning variable regions from B-cells Transient expression of recombinant antibodies 8-13 weeks Phase IV: Sequencing, Expression, and Purification […]
